- Title
- High-fat feeding redirects cytokine responses and decreases allergic airway eosinophilia
- Creator
- de Vries, A.; Hazlewood, L.; Fitch, P. M.; Seckl, J. R.; Foster, P.; Howie, S. E. M.
- Relation
- Clinical & Experimental Allergy Vol. 39, Issue 5, p. 731-739
- Publisher Link
- http://dx.doi.org/10.1111/j.1365-2222.2008.03179.x
- Publisher
- Wiley-Blackwell Publishing
- Resource Type
- journal article
- Date
- 2009
- Description
- Background: Dietary fat intake has been associated with obesity and obesity in its turn with attenuated airway function and asthma, but it is unclear whether or how high-fat intake per se alters immune function relevant to development of allergic asthma. Objective: To use a non-obese mouse model of mild to moderate allergic asthma to compare effects of high-fat with isocaloric control-diet on allergic immune responses. Methods: C57BL/6 mice weaned and maintained on control (11% fat calories) or isocaloric high-fat diet (58% fat calories) were systemically sensitized with ovalbumin and challenged in the lungs. Allergic airway inflammation was assessed by measuring lung inflammation; serum antibodies; and, cytokines in serum, bronchoalveolar lavage (BAL) fluid and in supernatants of in vitro stimulated lung draining lymph node and spleen lymphocytes. Results: There was a significant reduction in lung eosinophilia and IL-5 in high-fat fed mice. Lung draining lymph node cells from these mice showed reduced pro-inflammatory cytokine (MCP-1 and TNF-α) release after ovalbumin re-stimulation and reduced release of IL-13 after concanavalin-A stimulation, indicating a general rather than just an antigen-specific change. There was no difference in IFN-γ release. In contrast, pro-inflammatory cytokine release was increased from splenocytes. Decreased eosinophilia was not due to increased regulatory T cell or IL-10 induction in draining lymph nodes or spleen, nor to changes in antibody response to ovalbumin. However, decreased levels of serum and BAL eotaxin were found in high-fat fed animals. Conclusions: The data indicate that high-fat dietary content redirects local immune responses to allergen in the lungs and systemic responses in the spleen and serum. These effects are not due to changes in regulatory T cell populations but may reflect a failure to mobilize eosinophils in response to allergic challenge.
- Subject
- allergens; cytokines; diet; eosinophilia; eotaxin; fat; immunity; inflammation; lungs
- Identifier
- uon:7315
- Identifier
- http://hdl.handle.net/1959.13/807152
- Identifier
- ISSN:1365-2222
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